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采用美國Sage Science公司的Pippin Prep和Blue Pippin 全自動核酸電泳和片段回收系統發表的文章
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下列發表的文章采用美國Sage Science公司的Pippin Prep和Blue Pippin 全自動核酸電泳和片段回收系統或者從采用其技術或材料：
環亞生物科技有限公司（APG BIO Ltd）是美國Sage Science公司在中國大陸的總代理商，詳情請登錄www.apgbio.com
August 2013
De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana
Authors:
Stephen M Gross, Jeffrey A Martin, June Simpson, María Jazmín Abraham-Juarez, Zhong Wang and Axel Visel
Info:
Agave is an interesting plant for bioenergy study. This paper provides transcriptome data of two strains. The Pippin Prep was used to size selec cDNAs at 250 or 500 bp, followed by TrueSeq library construction and sequencing on the Illumina HiSeq.
Citation:BMC Genomics 2013 14:563
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August 2013
Comparative genomics of Campylobacter concisus isolates reveals genetic diversity and provides insights into disease association
Authors:
Nandan P Deshpande, Nadeem O Kaakoush, Marc R Wilkins and Hazel M Mitchell
Info:
Collaborators at the University of New South Wales sequence several strains of Campylobacter concisus from healthy and diseased (Crohn’s disease and gastroenteritis) individuals for a comparative study. Results showed genetice diversity and identified pathways that may be associated with the diseases. Pippin Prep was used to size selec libraries for Illumina HiSeq.
Citation:BMC Genomics 2013 14:585
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July 2013
Phylogeographic Origin of Helicobacter pylori Determines Host-Adaptive Responses upon Coculture with Gastric Epithelial Cells
Authors:
Alexander Sheh, Rupesh Chaturvedi, D. Scott Merrell, Pelayo Correa, Keith T. Wilson, James G. Fox
Info:
A study of gene expression comparing several strains of H. pylori. The BluePippin was used to size-selec fragments to construct paired-end libraries for Illumina MiSeq.
Citation:Infection and Immunity, July 2013 Volume 81 Number 7
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June 2013
The new sequencer on the block: comparison of Life Technology’s Proton sequencer to an Illumina HiSeq for whole-exome sequencing
Authors:
Joseph F. Boland, Charles C. Chung, David Roberson, Jason Mitchell, Xijun Zhang, Kate M. Im, Ji He, Stephen J. Chanock, Meredith Yeager, Michael Dean
Info:
An exome-sequencing study from the NIH comparing Illumina/Nimblegen to Ion Torrent/TargetSeq. Pippin size selection was used for library construction for Ion Proton sequencing.
Citation:Hum Genet (2013) 132:1153–1163
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June 2013
Skin Microbiome Imbalance in Patients with STAT1/STAT3 Defects Impairs Innate Host Defense Responses
Authors:
Sanne P. Smeekens, c Curtis Huttenhower, Anca Riza, Frank L. van de Veerdonk, Patrick L.J.M. Zeeuwen, Joost Schalkwijk, Jos W.M. van der Meer, Ramnik J. Xavier, Mihai G. Netea, Dirk Gevers
Info:
Genomic DNA was subjected to 16S PCR amplifications. Pippin Prep was used to retrieve the amplicons in order to isolate fragments away from aspecific products, prior to Illumina MiSeq sequencing.
Citation:J Innate Immun
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May 2013
Characterizing and measuring bias in sequence data
Authors:
Michael G Ross, Carsten Russ, Maura Costello, Andrew Hollinger, Niall J Lennon, Ryan Hegarty, Chad Nusbaum and David B Jaffe
Info:
Researchers at the Broad Institute compare sequence bias on Illumina, Ion, PacBio, and Complete Genomics platforms. Pippin sizing was used on the Ion Torrent libraries. The authors note that library construction conditions significantly influence sequencing bias.
Citation:Genome Biology 2013, 14:R51
