Plasmid DNA Purification Services
Let us take care of your plasmid DNA purification, so you can take care of your research. Ultrapure, low-endotoxin plasmid DNA for in vivo, in vitro, and preclinical applications:

High quality plasmid purifications, customized for your specific need
Stringent quality control ensure reliable results with every plasmid DNA sample
Ready-to-use plasmid DNA in about 7 days

Want to save time on basic experimental steps? We can perform your plasmid DNA purification at any scale you need, so you can concentrate on the wider scope of your research. Our new Plasmid DNA Purification Service produces high purity plasmid DNA for research or preclinical applications. All purified DNA undergoes vigorous testing to ensure that you receive the amount of quality DNA you need.
 
What we can do for you
Using a proprietary anion-exchange resin with an endotoxin removal process, Invitrogen’s Plasmid DNA Purification Service will improve your success in downstream applications by using the highest quality plasmids. Whether you require purification of one sample or hundreds, need 10 or 100 mg of ultrapure plasmid DNA, or want ultralow endotoxin levels, we’ll meet your needs and your timelines.

Basic Plasmid DNA Purification services include:

Standard yield Plasmid DNA construct
Includes production, purification, and quality control testing of standard yielding plasmid DNA, with final guaranteed yield up to 100mg based on plasmid copy number and culture volume. Standard yield constructs result in >3 mg plasmid DNA per liter of LB culture in shake flask. Should the actual yield be lower or the resulting plasmid be found unreasonably unstable, production will stop while the client is given options on how to proceed. This service provides a pre-screening process for host strain selection and optimization, if none are recommended by the client. This may also include testing of different host strains and growth conditions (temperatures, times and media) for plasmid integrity and highest yields. Plasmid purification process includes removal of endotoxins to levels at or below 100 EU/mg as confirmed by semi-quantitative assay on final product during standard QC analysis. Plasmid concentration, current buffer and method of preparation will be noted.

Low yield Plasmid DNA construct
Includes production, purification, and quality control testing of low yielding plasmid DNA (including low copy number plasmids, unstable and viral constructs), with final guaranteed yields up to 100mg based on plasmid copy number and culture volume.  Low yield constructs result in 1-3 mg plasmid DNA per liter of LB culture in shake flask. Should the actual yield be lower or the resulting plasmid be found unreasonably unstable, production will stop while the client is given options on how to proceed. This service provides a pre-screening process for host strain selection and optimization as listed above for standard yield constructs. This service also includes additional cultures and growth conditions to give the guaranteed yields using these low yielding constructs. Purification process includes removal of endotoxins to levels at or below 100 EU/mg as confirmed by semi-quantitative assay on final product during standard QC analysis. Plasmid concentration, current buffer and method of preparation will be noted.

Plasmid Purification from a 1L prep culture
Includes production, purification, and quality control testing of plasmids grown under standard conditions (37°C for 14-16 hours while shaking) in 1 L of LB or equivalent. These preps do not include screening of host strains or harvested bacteria from scale-up. There is no guaranteed minimum yield as the preparation is plasmid-dependent. However, most plasmids typically yield between 1-5 mg. Plasmid purification process includes removal of endotoxins to levels at or below 100 EU/mg.  Endotoxin levels will not be tested during standard QC analysis unless client selects the option to purchase a quantitative endotoxin test. Client will receive a certificate of conformance from QC analysis. Client will provide glycerol stock as starting material.

BAC purification from 1L and 5L prep cultures
Includes production, purification,n and quality control testing of plasmids grown under standard conditions (37°C for 14-16 hours while shaking) in 1 L or 5 L of LB, or equivalent. There is no guaranteed minimum yield as the preparation is plasmid-dependent. For QC analysis, restriction enzyme digestion information should be provided for sizing purposes for which a restriction enzyme digest charge will apply. Client will be provided with a certificate of conformance from QC analysis.

Invitrogen公司是在生命科學(xué)領(lǐng)域內(nèi)最主要的供貨商，其產(chǎn)品覆蓋了自分子生物學(xué)研究、蛋白質(zhì)表達(dá)、細(xì)胞培養(yǎng)、生物信息學(xué)、熒光檢測(cè)及藥品開發(fā)與篩選等生物工程的全過(guò)程。

自1987年成立以來(lái)，Invitrogen公司就一直致力于為生物科研及生產(chǎn)領(lǐng)域的客戶提供最有效的工具，包括各種便于使用的試劑盒與消耗品，我們的目標(biāo)是為客戶提供更快捷、更可靠、更有效的產(chǎn)品及服務(wù)。

在2000年度，在為生命科學(xué)領(lǐng)域提供產(chǎn)品和服務(wù)的行業(yè)內(nèi)最大的新聞，莫過(guò)于Life Technologies與Invitrogen的合并。Life Technologies 曾于1983年合并了著名的血清、培養(yǎng)基生產(chǎn)廠商GIBCO和分子生物學(xué)產(chǎn)品供貨商BRL。而成立于1987年的INVITROGEN更是以其銳意進(jìn)取的精神在短暫的十余年間迅速發(fā)展并連續(xù)并購(gòu)了NOVEX，RESEARCH GENETICS和SERVA ELECTROPHORESIS，近期更連續(xù)收購(gòu)了三家在生物產(chǎn)品的不同領(lǐng)域都舉足輕重的公司：InforMax,PanVera,Molecular Probes，使得我們的產(chǎn)品進(jìn)一步擴(kuò)大到了生物信息學(xué)、新藥研發(fā)與篩選及熒光檢測(cè)。最終合并后的公司總稱INVITROGEN。我司的全部產(chǎn)品均符合ISO9001認(rèn)證，并在cGMP標(biāo)準(zhǔn)條件下生產(chǎn)。

以下為invitrogen中國(guó)區(qū)辦事處及授權(quán)經(jīng)銷商的聯(lián)系方式，請(qǐng)根據(jù)您所在區(qū)域從invitrogen公司授權(quán)的指定經(jīng)銷商選購(gòu)我們的產(chǎn)品！

各地代理商聯(lián)系方式：
中國(guó)地區(qū)授權(quán)經(jīng)銷商：
北京九宇金泰生物技術(shù)有限公司 (北京、天津、吉林、黑龍江、山東、遼寧、河南、山西、內(nèi)蒙古、新疆、甘肅、山西、寧夏、青海、陜西 )
電話:010-87388325、87388326、87388328

上海禾晟生化科技有限公司 （上海、江西、江蘇，浙江，安徽）
電話：021-64745426

武漢佰歐樂基生物科技有限公司(湖北)
電話：027-87165982

廣州英韋創(chuàng)建生物科技有限公司（廣東、湖南、廣西、海南、云南、福建、四川）
電話：400-678-6369 020-28867866

重慶市百瑞生物工程有限公司（重慶、貴州）
電話：023-65463498

Invitrogen中國(guó)地區(qū)辦事處 www.invitrogen.com
E-mail:sales-cn@invitrogen.com

技術(shù)服務(wù)熱線：800 820 8982；400 820 8982　　
技術(shù)服務(wù)郵箱：cntechsupport@invitrogen.com

invitrogen上海辦事處　　電話：021-61452000　傳真：021-6448 2550
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